HIPREP 16/60 SEPHACRYL S-300 HR

Details

HiPrep Sephacryl S-300 HR columns are designed for routine size exclusion chromatography of proteins, such as antibodies and serum proteins.

Wide fractionation rangeÂ â€“ M_rÂ 10 000â€“1 500 000 for globular proteins, well suited for antibody purification
High capacity columnsÂ â€“ preparative separation of proteins in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively
ConvenientÂ â€“ prepacked Sephacryl columns are easily connected to KTA systems for automated chromatography at low to medium pressure

Successful use in published literature

Sephacryl S-300 HR resin has been used for decades to separate proteins by size exclusion chromatography (SEC; also called gel filtration). Here are a few of the proteins that have been purified using these Sephacryl columns:

IntactÂ complement factor HÂ from human plasma
AnÂ antibody-dendrimer conjugateÂ functionalized with a targeting agent
Soybean mature glycininÂ A1bB2

Other S-300 HR resin formats

Sephacryl S-300 HR is also available as bulk resin for packing columns of your own.

Other Cytiva columns for SEC (gel filtration)

Life Sciences solutions from Cytiva include a comprehensive range of size exclusion chromatography products, such as prepacked columns and loose resin. For higher resolution, HiLoad Superdex 200 pg columns are available. Not sure which product to use Let Cytiva help youÂ find the right SEC resin or columnÂ for your needs.

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