Welcome to the Refreshed Webstore!Click for more details.
 

HISTRAP FF 5 X 5 ML

SKU
GE/17525501
Brand
CYTIVA
Pre-order (Deliver in 8 to 12 weeks)
Login For Price

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

  • High binding capacity, approx. 40 mg/mL resin.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.
More Information
Brand CYTIVA
Write Your Own Review
You're reviewing:HISTRAP FF 5 X 5 ML
Your Rating
Who Viewed This Also Viewed
  1. GD/X Syringe Filter PES 25mmx0.45um/50 Sterile
    GD/X Syringe Filter PES 25mmx0.45um/50 Sterile Brand: WHATMAN SKU: WH/MI/BO/68962504
    Login For Price
  2. 8-channel VOYAGER Electronic Pipette, 0.5 - 12.5 µl
    8-channel VOYAGER Electronic Pipette, 0.5 - 12.5 µl Brand: INTEGRA BIOSCIENCES SKU: IB/4721
    Login For Price
  3. KING B MEDIUM (PSEUDOMONAS F AGAR) (USP) 500 grams/bottle
    KING B MEDIUM (PSEUDOMONAS F AGAR) (USP) 500 grams/bottle Brand: CONDALAB SKU: CDL/1532
    Login For Price
  4. Mini-UniPrep Filter 0.45um NYL/1000
    Mini-UniPrep Filter 0.45um NYL/1000 Brand: WHATMAN SKU: WH/MI/BO/UN503NPUNYL
    Login For Price
  5. Nylon Membrane 0.45umx47mm/100
    Nylon Membrane 0.45umx47mm/100 Brand: WHATMAN SKU: WH/MI/AO/7404004
    Login For Price