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Vivaspin 2 3 kDa MWCO PES 25/pk

SKU
GE/28932240
Brand
CYTIVA
Pre-order (Deliver in 8 to 12 weeks)
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-ivaspin protein concentrator spin columns

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-ivaspinâ„¢ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.

- Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.  
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  • -strong>-ase-of-use:- One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
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  • -strong>-ample protection:- Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
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  • -strong>-ase-of-use:- One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
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  • -strong>-igh flow rates:- And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
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  • -strong>-inimal protein adsorption:- Thanks to the Vivaspinâ„¢ polycarbonate tube design.
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  • -strong>-ide selection:- Vivaspin sample concentrators are suitable for sample volumes ranging from 100 μL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.
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  • -ownload the Vivaspin protein concentrator data file to learn more and to determine the right MWCO (molecular weight cut-off) and volume for your enrichment study.
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-pin column protein concentration – how does it work

- Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.

-pplications for ultrafiltration spin columns

- Vivaspin ultrafiltration spin columns are a rapid and flexible solution for protein concentration and small-scale preparation of biological molecules directly from cell lysates and biological fluids, following upstream purification steps. They can also be used prior to downstream analyses, such as gel electrophoresis, liquid chromatography (LC), mass spectrometry (MS), and LC-MS. Examples include:
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  • -oncentration of urine prior to electrophoresis for identification and diagnosis of multiple myeloma and related serum protein disorders.
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  • -oncentration of recombinant human IFN-g following purification from culture supernatant.
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- Effective enrichment of low-M(r) components from human plasma for proteomic analysis to identify plasma-based biomarkers in disease.
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Brand CYTIVA
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